Rhesus DKK-1 (256 Asn/Gln, His Tag) recombinant protein

Rhesus DKK-1 (256 Asn/Gln, His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Dickkopf (DKK) family proteins, consisting of DKK-1, DKK-2, DKK-3 and DKK-4, function as secreted Wnt antagonists by inhibiting Wnt coreceptors LRP5/6. DKK-1, DKK-2, and DKK-4 also bind cell surface Kremen-1 or Kremen-2 and promote the internalization of LRP5/6. Dickkopf related protein 1 (DKK-1) was initially identified as an inducer of head formation in Xenopus embryos. DKK-1 protein modulates Wnt signaling pathway during embryonic development. Increased levels of DKK-1 are found in the majority of lung cancers, esophageal squamous cell carcinomas, and hormone-resistant breast cancers, while DKK-1 expression is decreased in malignant melanoma and colorectal cancers.

DKK-1; MDKK-1; DICKKOPF 1; SK; HDKK 1; S; DKK1

Human Cells

A DNA sequence encoding the rhesus DKK1 (XP_001098844.1) precursor (Met 1-His 266) (256 Asn / Gln) was expressed, fused with a C-terminal polyhistidine tag.

Rhesus

The recombinant rhesus DKK1 comprises 246 amino acids with a predicted molecular mass of 27.2 kDa. As a result of glycosylation, the apparent molecular mass of rhesus DKK1 is approximately 38-43 kDa in SDS-PAGE under reducing conditions.

> 97 % as determined by SDS-PAGE

Measured by its ability to inhibit Wnt3a induced alkaline phosphatase production by C3H10T1/2 2A6 mouse embryonal fibroblast cells. The ED₅₀ for this effect is typically 0.5-3 μg/ml in the presence of 10 ng/mL of Wnt3a.