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Product Name
Mouse Renin (His Tag) recombinant protein
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Description
Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney.
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Protein name
Renin-1
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Protein short names
REN1D; REN-A; RN-1; FLJ10761; REN; HNFJ2; D19352; RNR; REN1C; RENIN; REN-1
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Uniprot ID
P06281
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Gene Name
Ren1; Ren; Ren-1
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Source/Expression Host
Human Cells
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Expression Plasmid/cDNA
A DNA sequence encoding the pro form of mouse REN1 (NP_112469.1) (Met 1-Arg 402) was fused with a polyhistidine tag at the C-terminus.
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Protein Species
Mouse
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Molecular weight
The recombinant mouse REN1 consists of 392 amino acids and has a predicted molecular mass of 43.2 kDa. As a result of glycosylation, the apparent molecular mass of rmREN1 is approximately 48-55 kDa in SDS-PAGE under reducing conditions.
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Purity
> 97 % as determined by SDS-PAGE
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Activity
1. Measured by its ability to cleave the fluorogenic peptide substrate 5FAM/QXL™ 520 (PetiPeterdi, J. et al., 2009, Physiology 24:88.). The specific activity is > 20 pmoles/min/μg.
2. Immobilized mouse REN1-His (Cat: 502823) at 10ug/ml (100 μl/well) can bind biotinylated human AGT-His (Cat: 501652) with a linear range of 31.25-250 ng/ml. -
Validations
Mouse REN1 / Renin-1 Protein (His Tag) SDS-PAGE
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