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Product Name
Mouse REG4 (His Tag) recombinant protein
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Description
Calcium-independent lectin displaying mannose-binding specificity and able to maintain carbohydrate recognition activity in an acidic environment. May be involved in inflammatory and metaplastic responses of the gastrointestinal epithelium (By similarity).
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Protein name
Regenerating islet-derived protein 4
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Protein short names
GISP; RELP; REG-4; REG-IV; 2010002L15RIK; REG4
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Uniprot ID
Q9D8G5
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Gene Name
Reg4
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Source/Expression Host
Human Cells
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Expression Plasmid/cDNA
A DNA sequence encoding mouse REG4 (NP_080604.2) (Met 1-Thr 157) was expressed with the polyhistide tag at the C-terminus.
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Protein Species
Mouse
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Molecular weight
The recombinant mouse REG4 comprises 146 amino acids and has a predicted molecular mass of 17.5 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm REG4 is approximately 23-30 kDa due to glycosylation.
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Purity
> 95 % as determined by SDS-PAGE
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Activity
Measured by the ability of the immobilized protein to support the adhesion and proliferation of HCT-116 human colorectal carcinoma cells (ATCC: CCL-247) under low serum conditions. When 5 x 10E4cells/well are added to mouse REG4 coated plates (0.16 μg/ml and 100 μl/well), >70% cell will adhere after 60 minutes at 37℃.
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Validations
Mouse REG4 / GISP / RELP Protein (His Tag) SDS-PAGE
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