Recombinant DT3C (Diphtheria toxin & spg 3C domain) protein

Recombinant DT3C (Diphtheria toxin & spg 3C domain) protein

• DataSheet
• Material Safety Data Sheets (MSDS)

DT3C is a powerful tool used in the field of antibody-drug conjugates (ADC) for in vitro drug internalization. This is particularly critical in the field of drug development where understanding how antibodies are internalized by cells can influence the efficacy of the drug. Incomplete statistics reveal that DT3C has been utilized in nearly 130 ADC drug mAb screening patents as a tool to detect the ability of cells for mAb internalization. The DT3C protein is composed of two parts: 1. Diphtheria toxin (DT) lacking the receptor-binding domain but containing the C1, C2, and C3 domains. The DT component is a potent toxin that can inhibit protein synthesis and cause cell death when internalized; 2. The C1, C2, and C3 domains of Streptococcus protein G (3C), which can bind to the Fc portion of IgG antibodies. When a monoclonal antibody is binding with DT3C, the resulting mAb-DT3C complex can be used to assess the ability of the antibody to be internalized by cells. If the mAb-DT3C conjugate reduces the viability of cancer cells, it indicates that the monoclonal antibody has been successfully internalized, making it a potential candidate for ADC development.

E. coli

DNAs encoding 33-417aa (P00588) & 291-497aa (P19909) were fused with N-terminal 6His tag

>90%, by SDS-PAGE with Coomassie Brilliant Blue staining.