Recombinant RBM20 protein (His tag)

• DataSheet
• COA (Batch NP456393)
• Material Safety Data Sheets (MSDS)

Recombinant Human RBM20 (517-625) Fragment: A high-purity, functional protein fragment encompassing the RNA Recognition Motif (RRM) domain of RNA-binding protein 20. This product is an essential tool for investigating the molecular mechanisms of alternative splicing regulation, particularly in cardiac and skeletal muscle development.

RNA-binding protein 20 (RBM20) is a critical regulator of mRNA splicing for genes encoding key structural proteins involved in cardiac development, such as TTN (Titin), CACNA1C, and CAMK2D. It functions as a splicing repressor by binding the 5'UCUU-3' motif in pre-mRNAs, leading to exon exclusion in target transcripts. RBM20-mediated exon skipping is hormone-dependent and vital for TTN isoform transition in muscle tissues, and it also facilitates the formation of circular RNAs from TTN transcripts. The protein localizes primarily to the nucleus in its active form, where it regulates alternative splicing, but it also resides in cytoplasmic ribonucleoprotein granules, with its subcellular distribution modulated by phosphorylation. The provided recombinant fragment (517-625) contains the RRM domain, enabling focused studies on RNA-binding specificity and splicing regulatory functions.

MGRVVHICNL PEGSCTENDV INLGLPFGKV TNYILMKSTN QAFLEMAYTE AAQAMVQYYQ EKSAVINGEK LLIRMSKRYK
ELQLKKPGKA VAAIIQDIHS QRERDMFREA LEHHHHHH