Rb9 peptide
Not For Human Use, Lab Use Only.
Cat.#: 319247
Special Price 152.9 USD
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Product Name
Rb9 peptide
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Documents
Batch to batch variation of the purity
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Sequence Shortening
H-CARGETARATFAYWGQGC-NH2
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Sequence
H-Cys-Ala-Arg-Gly-Glu-Thr-Ala-Arg-Ala-Thr-Phe-Ala-Tyr-Trp-Gly-Gln-Gly-Cys-NH2
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Length (aa)
18
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Peptide Purity (HPLC)
95.35%
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Molecular Formula
C83H123N27O24S2
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Molecular Weight
1947.15
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Source
Synthetic
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Form
Powder
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Description
Peptide Rb9 is a cyclic peptide derived from the VHCDR3 of a humanized monoclonal antibody called RebmAb 200. It reacts with an epitope in the sodium-phosphate transporter NaPi2b and displays bio-reactivity independent of mAb specificity. The peptide was synthesized by Peptide 2.0 and modifications included amidation at the C terminus (NH2) and cyclization (C-C, disulfide bridge). It has been shown to have immunomodulatory protective effects in a metastatic melanoma setting and has been tested against syngeneic CT26 colon and Panc02 pancreatic cancer cells grafted subcutaneously in C57Bl/6 mice. It activates murine or human dendritic cells and enhances the immune response against cancer cells. The peptide has been studied for its anti-tumor protective effects against metastatic melanoma and has shown potential as a new anticancer drug.
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Storage Guidelines
Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides
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References
- Machado FC, Girola N, Maia VSC, Bergami-Santos PC, Morais AS, Azevedo RA, Figueiredo CR, Barbuto JAM, Travassos LR. Immunomodulatory Protective Effects of Rb9 Cyclic-Peptide in a Metastatic Melanoma Setting and the Involvement of Dendritic Cells. Front Immunol. 2020 Jan 15;10:3122. doi: 10.3389/fimmu.2019.03122. PMID: 32010152; PMCID: PMC6974543.
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About TFA salt
Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.
TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.
TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.
In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.
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Molar Concentration Calculator
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Dilution Calculator
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Percent Concentration Calculator
Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)
Related Products / Services
• Peptide Services: NovoPro's peptide synthesis services include standard chemical peptide synthesis, peptide modification, peptide libraries, and recombinant peptide expression.
• Standard Peptide Synthesis: NovoPro offers quality peptides at the most competitive prices in the industry, starting at $3.20 per amino acid. NovoPro provides PepBox – Automatic Quote Tool for online price calculation.
• Peptide Modifications: NovoPro offers a wide range of peptide modification services including isotope labeling (2H, 15N, and 13C), multiple disulfide bonds, multiple phosphorylations, KLH, BSA, ovalbumin, amidation, acetylation, biotin, FITC, etc.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"