Rat TNFSF12 (Fc Tag) recombinant protein

Rat TNFSF12 (Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

TNFSF12 is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. It is a ligand for the FN14/TWEAKR receptor. TNFSF12 has overlapping signaling functions with TNF, but displays a much wider tissue distribution. It can induce apoptosis via multiple pathways of cell death in a cell type-specific manner. It is also found that TNFSF12 promotes proliferation and migration of endothelial cells, and thus acts as a regulator of angiogenesis. TNFSF12 also is a weak inducer of apoptosis in some cell types and mediates NF-kappa-B activation.

TWEAK; MEMBER 12; APO3L; TNFSF12; DR3LG; APO3 LIGAND; TNF-RELATED WEAK INDUCER OF APOPTOSIS; RP23-422L16.25-001; DR3L; TUMOR NECROSIS FACTOR LIGAND SUPERFAMILY MEMBER 12; APO3/DR3 LIGAND; TUMOR NECROSIS FACTOR (LIGAND) SUPERFAMILY; UNQ181/PRO207

Human Cells

A DNA sequence encoding the rat TNFSF12 (Arg105-His249) was expressed with Fc region of human IgG1 at the N-terminus.

Rat

The recombinant rat TNFSF12 comprises 405 amino acids and predicts a molecular mass of 44.4 kDa. The apparent molecular mass of the recombinant protein is approximately 49 kDa in SDS-PAGE under reducing conditions due to glycosylation.

> 85 % as determined by SDS-PAGE

1. Measured in a cell proliferation assay using HUVEC human umbilical vein endothelial cells. The ED₅₀ for this effect is typically 5-20 ng/mL.
2. Immobilized Fc-rat TNFSF12 at 10 μg/ml (100 μl/well) can bind biotinylated human Fc-TNFRSF12A (Cat:502881), The EC₅₀ of biotinylated human Fc-TNFRSF12A (Cat:502881) is 0.12-0.28 μg/ml.