Rat IL-6 recombinant protein

Rat IL-6 recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Interleukin-6 (IL-6) is a multifunctional α-helical cytokine that regulates cell growth and differentiation of various tissues, which is known particularly for its role in the immune response and acute phase reactions. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. It exerts actions through the its heterodimeric receptor composed of IL-6R that lacks the tyrosine/kinase domain and binds IL-6 with low affinity, and ubiquitously expressed glycoprotein 130 (gp130) that binds the IL-6. IL-6R complex with high affinity and thus transduces signals. IL-6 is also involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.

IL6; HSF; BSF2; HGF; IL-6; IFNB2

E. coli

A DNA sequence encoding the rat IL6 (Phe25-Thr211) was expressed and purified with an initial Met.

Rat

The recombinant rat IL6 consists of 188 amino acids and has a predicted molecular mass of 21.9 kDa. As a result of glycosylation, the apparent molecular mass of the recombinant protein is approximately 22 kDa in SDS-PAGE under reducing conditions.

> 90 % as determined by SDS-PAGE

Measured in a cell proliferation assay using T1165 mouse plasmacytoma cells.
The ED₅₀ for this effect is typically 1-6 ng/ml.