Rat IFNA5/IFNaG (His Tag) recombinant protein

Rat IFNA5/IFNaG (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Interferon, alpha 5 (IFNA5) belongs to the alpha/beta interferon family. IFNA5 is the only IFNA subtype detected in normal liver, while a mixture of subtypes is observed in the liver tissue of patients with chronic hepatitis C. Interferons are produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase. IFN-alpha, the first cytokine to be produced by recombinant DNA technology, has emerged as an important regulator of growth and differentiation, affecting cellular communication and signal transduction pathways as well as immunological control. Originally discovered as an antiviral substance, the efficacy of IFN-alpha in malignant, viral, immunological, angiogenic, inflammatory, and fibrotic diseases suggests a spectrum of interrelated pathophysiologies. IFN-alpha emerged as a prototypic tumor suppressor protein that represses the clinical tumorigenic phenotype in some malignancies capable of differentiation.

RP23-139P14.29; RP11-380P16.5; IFA5; IFNA5

Human Cells

A DNA sequence encoding the rat IFNA5 (XP_001076062.1) (Met1-Glu189) was expressed, fused with a polyhistidine tag at the C-terminus.

Rat

The recombinant rat IFNA5 comprises 179 amino acids and predicts a molecular mass of 20.9 kDa. The apparent molecular mass of the recombinant protein is approximately 21 kDa in SDS-PAGE under reducing conditions due to glycosylation.

> 95 % as determined by SDS-PAGE

Measured in antiviral assays using L929 cells infected with vesicular stomatitisvirus (VSV).
The ED₅₀ for this effect is typically 10-50 pg/mL.