Rat HGF / Hepatocyte Growth Factor recombinant protein

Rat HGF / Hepatocyte Growth Factor recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Hepatocyte growth factor, also known as HGF, contains 4 kringle domains, 1 PAN domain and 1 peptidase S1 domain. It belongs to the peptidase S1 family, plasminogen subfamily. Hepatocyte growth factor is secreted by mesenchymal cellsas a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor, and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. HGF is a potent mitogen for mature parenchymal hepatocyte cells, seems to be an hepatotrophic factor, and acts as growth factor for a broad spectrum of tissues and cell types. HGF has no detectable protease activity. Defects in hepatocyte growth factor are the cause of deafness autosomal recessive type 39. A form of profound prelingual sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information.

C230052L06RIK; F-TCF; SF/HGF; NK1; SF; HGFB; NK2; HGF/SF; HGF; DFNB39; HPTA

Human Cells

A DNA sequence encoding the rat HGF (Met1-Leu728) was expressed and purified.

Rat

The recombinant rat HGF consists of 698 amino acids and has a predicted molecular mass of 79.5 kDa. As a result of glycosylation, the apparent molecular mass of the recombinant protein is approximately 92 kDa in SDS-PAGE under reducing conditions.

> 95 % as determined by SDS-PAGE

Measured by its ability to neutralize TGF-beta mediated inhibition on Mv-1-Lu cell proliferation.
The ED₅₀ for this effect is typically 0.2-1 ng/mL.