Rat CD5 / Cluster of Differentiation 5 (His Tag) recombinant protein

Rat CD5 / Cluster of Differentiation 5 (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD5 is a member of the CD system. CD5 was found to be widely distributed in T-cells and B1 cells which is a subset of IgM-secreting B cells. CD5 also was found expressed in small lymphocytic lymphoma, hairy cell leukaemia and mantle cell lymphoma cells. CD5 serves to weaken the activating stimulus from the BCR so that the B1 cells can only reflect to the very strong stimuli but not the normal tissue proteins.

T-cell surface glycoprotein CD5

LYT-1; CD5; LY-12; LY-A; LY-1; T1; LEU1

P51882

Cd5

Human Cells

A DNA sequence encoding the rat CD5 (P51882) (Met1-Pro368) was expressed, fused with a polyhistidine tag at the C-terminus.

Rat

The recombinant rat CD5 comprises 356 amino acids and predicts a molecular mass of 38.9 kDa. The apparent molecular mass of the recombinant protein is approximately 46-49 kDa in SDS-PAGE under reducing conditions due to glycosylation.

> 95 % as determined by SDS-PAGE

Immobilized rat CD5-His at 10 μg/ml (100 μl/well) can bind biotinylated human GST-LCK (Cat:500189), The EC₅₀ of biotinylated human GST-LCK (Cat:500189) is 0.9-2.1 μg/ml.