Rat CD16a/FCGR3A (His Tag) recombinant protein

Rat CD16a/FCGR3A (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

The Fc receptor with low affinity for IgG (FCGR3, or CD16) is encoded by 2 nearly identical genes, FCGR3A and FCGR3B, resulting in tissue-specific expression of alternative membrane-anchored isoforms. FCGR3A, it is also known as CD16a, encodes a transmembrane protein expressed on activated monocytes/macrophages, natural killer (NK) cells, and a subset of T cells.\CD16a / FCGR3A is a receptor expressed on NK cells that facilitates antibody dependent cellular cytotoxicity (ADCC) by binding to the Fc portion of various antibodies. CD16a / FCGR3A also has a broader function. CD16a / FCGR3A is directly involved in the lysis of some virus-infected cells and tumor cells by NK cells, independent of antibody binding. Cross-linking of CD16a / FCGR3A on NK cells resulted in increased intracellular Ca2+ levels and a cascade of biochemical events similar to those activated by the T cell receptor. CD16a / FCGR3A on human NK cells is a lysis receptor that mediates the direct killing of some virus infected and tumor cells, independent of antibody ligation. \

RP11-5K23.1; CD16; FCRIIIA; FCRIII; FCGRIV; FCR-10; CD16A; FCGAMMARIV; 4833442P21RIK; IMD20; FCGR3; FCGR3A; FCGRIII; IGFR3; CD16-2; FCRL3; FCG3

Human Cells

A DNA sequence encoding the rat FCGR3A (ABO70343.1) extracellular domain (Met 1-Pro 200) was expressed, fused with a polyhistidine tag at the C-terminus.

Rat

The recombinant rat FCGR3A comprises 190 amino acids and predicts a molecular mass of 21.7 kDa. The apparent molecular mass of the rat FCGR3A is approximately 28-33 kDa in SDS-PAGE under reducing conditions due to glycosylation.

> 97 % as determined by SDS-PAGE

Measured by its binding ability in a functional ELISA.
Immobilized rat FCGR3A-His (Cat:504601) at 10 μg/ml (100 μl/well) can bind biotinylated human IgG1, The EC₅₀ of biotinylated human IgG1 is 59.0-139.0 ng/ml.