Rat CD153/CD30L/TNFSF8 (Fc Tag) recombinant protein

Rat CD153/CD30L/TNFSF8 (Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

CD30 ligand (CD30L), also known as CD153 and TNFSF8, is a membrane-associated glycoprotein belonging to the TNF superfamily and TNFR superfamily, and is a specific ligand for CD30/TNFRSF8 originally described as a cell surface antigen and a marker for Hodgkin lymphoma and related hematologic malignancies. CD30L is a type-II membrane glycoprotein expressed on activated T cells, stimulated monocyte-macrophages, granulocytes, eosinophils, and some Burkitt-like lymphoma cell lines. CD30L is capable of transducing signals through CD30 on different CD30+ lymphoma cell lines, and mediates pleiotropic biologic effects including cell proliferation, activation, differentiation, as well as cell death by apoptosis. CD30-CD30 ligand interaction has been suggested to have a pathophysiologic role in malignant lymphomas, particularly Hodgkin disease, large cell anaplastic lymphomas and Burkitt lymphomas, and is also involved in activation and functioning of the T cell-dependent immune response. Thus, CD153 and its receptor CD30 are regarded as therapeutic targets in hematologic malignancies, autoimmune and inflammatory diseases.

MGC138144; CD30L; TNFSF8; CD30LG; CD153

Human Cells

A DNA sequence encoding the rat TNFSF8 (Gln66-Asp237) was expressed with Fc region of human IgG1 at the N-terminus.

Rat

The recombinant rat TNFSF8 comprises 432 amino acids and predicts a molecular mass of 47.9 kDa. The apparent molecular mass of the recombinant protein is approximately 58 kDa in SDS-PAGE under reducing conditions due to glycosylation.

> 90 % as determined by SDS-PAGE

Immobilized rat S4-Fc3L3-TNFSF8 at 10 μg/ml (100 μl/well) can bind biotinylated human CD30-Fch (Cat:504541), The EC₅₀ of biotinylated human CD30-Fch (Cat:504541) is 8.3-19.3 ng/ml.