Rat Cadherin-8 / CDH8 recombinant protein

Rat Cadherin-8 / CDH8 recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Cadherins are integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Type I cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. The extracellular domain consists of five subdomains, each containing a cadherin motif, and appears to determine the specificity of the protein's homophilic cell adhesion activity. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I  cadherins. Cadherin 8, also known as CDH 8, is a type I I classical cadherin belonging to the cadherin superfamily. As mainly expressed in brain, CDH8 is found in certain nerve cell lines, such as retinoblasts, glioma cells and neuroblasts, and is putatively involved in synaptic adhesion, axon outgrowth and guidance. Human Cadherin 8 is a 799 amino acid single-pass type I  transmembrane protein with a putative 29 aa signal sequence, and a 32 aa propeptide, a 560 aa mature extracellular domain, a 21 aa transmembrane domain and a 157 aa cytoplasmic domain. The human, mouse and rat proteins share approximately 98% homology.

Cadherin 8

AI851472; CDH8; CADHERIN-8; NBLA04261; CAD8

F1LNN3

Cdh8; rCG_39042

Human Cells

A DNA sequence encoding the rat CDH8 (NP_445845.2)(Met1-Met621) was expressed with six amino acids (LEVLFQ) at the C-terminus.

Rat

The recombinant rat CDH8 comprises 599 amino acids and predicts a molecular mass of 65.9 kDa. The apparent molecular mass of the recombinant protein is approximately 76-85 kDa in SDS-PAGE under reducing conditions due to glycosylation.

> 80 % as determined by SDS-PAGE