Rat ALK-4 / ACVR1B (Fc Tag) recombinant protein

Rat ALK-4 / ACVR1B (Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

ALK-4 (Activin Receptor-Like Kinase 4) or ACVR1B (Activin A Receptor, type 1B), belongs to the protein kinase superfamily, TKL Ser/Thr protein kinase family, and TGFB receptor subfamily. ALK-4/ACVR1B acts as a transducer of activin or activin like ligands signals. Activin binds to either ACVR2A or ACVR2B and then forms a complex with ACVR1B. The known type II activin receptors include ActRII and ActRIIB, while the main type I activin receptor in mammalian cells is ALK-4 (ActRIB). In the presence of activin, type II and type I receptors form complexes whereby the type II receptors activate ALK-4 through phosphorylation. The activated ALK-4, in turn, transduces signals downstream by phosphorylation of its effectors, such as Smads, to regulate gene expression and affect cellular phenotype. ALK-4/ACVR1B is an important regulator of vertebrate development, with roles in mesoderm induction, primitive streak formation, gastrulation, dorsoanterior patterning, and left-right axis determination.

ALK4; ALK-4; 6820432J04; ACVR1B; ACTR-IB; SKR2; ACVRLK4; ACTRIB

Human Cells

A DNA sequence encoding the rat ACVR1B (Met1-Glu126) was expressed with the Fc region of human IgG1 at the C-terminus.

Rat

The recombinant rat ACVR1B/Fc is a disulfide-linked homodimer. The reduced monomer comprises 344 amino acids and has a predicted molecular mass of 38.4 kDa. The apparent molecular mass of the protein is approximately 42 and 34 kDa in SDS-PAGE under reducing conditions.

> 95 % as determined by SDS-PAGE

Measured by its ability to bind with rat TDGF1-His (Cat:504857) in a functional ELISA.