RANATUERIN 3 peptide

RANATUERIN 3 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

H-GFLDIINKLGKTFAGHMLDKIKCTIGTCPPSP-OH

H-Gly-Phe-Leu-Asp-Ile-Ile-Asn-Lys-Leu-Gly-Lys-Thr-Phe-Ala-Gly-His-Met-Leu-Asp-Lys-Ile-Lys-Cys-Thr-Ile-Gly-Thr-Cys-Pro-Pro-Ser-Pro-OH

32

95.69%

C₁₅₄H₂₅₁N₃₉O₄₂S₃

3417.06

Synthetic

Powder

RANATUERIN 3 is an antimicrobial peptide found in Rana catesbeiana (Lithobates catesbeianus, North American bull frog), and has antibacterial activity against gram-positive bacterium Staphylococcus aureus (MIC=60 µM). It shows no detectable hemolytic activity at aconcentration of 20µg/ml against human red blood cell.
RANATUERIN 3 is a cyclic peptide with an alpha helix structure and a disulfide bridge between Cys23 and Cys28. It belongs to the frog skin active peptide family (Brevinin subfamily).

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides

• Goraya J, Knoop FC, Conlon JM. Ranatuerins: antimicrobial peptides isolated from the skin of the American bullfrog, Rana catesbeiana. Biochem Biophys Res Commun. 1998;250(3):589-92.
• Mechkarska M, Ojo OO, Meetani MA, Coquet L, Jouenne T, Abdel-Wahab YH, Flatt PR, King JD, Conlon JM. Peptidomic analysis of skin secretions from the bullfrog Lithobates catesbeianus (Ranidae) identifies multiple peptides with potent insulin-releasing activity. Peptides. 2011 Feb;32(2):203-8.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.