RAGE antagonist peptide

RAGE antagonist peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

Acetylation-ELKVLMEKEL-NH2

Acetylation-Glu-Leu-Lys-Val-Leu-Met-Glu-Lys-Glu-Leu-NH2

10

95.44%

C₅₇H₁₀₁N₁₃O₁₇S

1272.55

Synthetic

Powder

RAGE antagonist peptide is an advanced glycation end products (RAGE) antagonist. RAP binds to the receptor for advanced glycation end products (RAGE) and blocks its activation by multiple ligands, including S100P, S100A4, and HMGB - 1. It inhibits the interaction of these ligands with RAGE in vitro and in vivo, reducing the ability of the ligands to stimulate RAGE activation of NFkB in cancer cells. RAP also shows the ability to inhibit tumor growth and metastasis in preclinical models of pancreatic cancer and glioma. RAGE antagonist peptide (RAP) possesses anti-tumor and anti-inflammatory activities.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Arumugam T, Ramachandran V, Gomez SB, Schmidt AM, Logsdon CD. S100P-derived RAGE antagonistic peptide reduces tumor growth and metastasis. Clin Cancer Res. 2012 Aug 15;18(16):4356-64. doi: 10.1158/1078-0432.CCR-12-0221. Epub 2012 Jun 20. PMID: 22718861; PMCID: PMC3845828.
• Yao L, Zhao H, Tang H, Liang J, Liu L, Dong H, Zou F, Cai S. The receptor for advanced glycation end products is required for β-catenin stabilization in a chemical-induced asthma model. Br J Pharmacol. 2016 Sep;173(17):2600-13. doi: 10.1111/bph.13539. Epub 2016 Jul 27. PMID: 27332707; PMCID: PMC4978160.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.