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Product Name
RACGAP1 antibody
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Description
RACGAP1 Rabbit Polyclonal antibody. Positive IP detected in mouse testis tissue, HeLa cells. Positive WB detected in K-562 cells, HeLa cells, mouse testis tissue, RAW 264.7 cells. Positive IF detected in HeLa cells. Positive IHC detected in human thymus tissue. Observed molecular weight by Western-blot: 71 kDa
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Tested applications
ELISA, IHC, WB, IP, IF
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
HsCYK 4 antibody; ID GAP antibody; KIAA1478 antibody; Male germ cell RacGap antibody; MgcRacGAP antibody; RACGAP1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of RACGAP1 recombinant protein (Accession Number: XM_006719359). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:114507(RACGAP1 Antibody) at dilution of 1:600
Immunohistochemical of paraffin-embedded human thymus using Catalog No:114507(RACGAP1 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human thymus using Catalog No:114507(RACGAP1 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of (-20oc Ethanol) fixed HeLa cells using Catalog No:114507(RACGAP1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
IP Result of anti-RACGAP1 (IP:Catalog No:114507, 4ug; Detection:Catalog No:114507 1:500) with mouse testis tissue lysate 4000ug.
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Background
Rac GTPase-activating protein 1 (RACGAP1), localized in the mitotic spindle, has guanosine triphosphatase-activating protein (GAP) activity for the Rho family GTPases. RACGAP1 has an important influence on the initiation of cytokinesis, control of cell growth and differentiation, regulation of spermatogenesis, and tumor metastasis.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"