Q11 peptide

Q11 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-QQKFQFQFEQQ-OH

H-Gln-Gln-Lys-Phe-Gln-Phe-Gln-Phe-Glu-Gln-Gln-OH

11

95.02%

C₆₈H₉₆N₁₈O₂₀

1485.59

Synthetic

Powder

Q11 is an self-assembling peptide, which assembles into nanofibers and hydrogels in physiological conditions. Epitope-bearing nanofibers can be formed by synthesizing Q11 peptides with various epitopes attached at their termini and allowing them to assemble, and these nanofibers are able to induce robust antigen-specific immune responses with minimal associated inflammation.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Wen Y, Waltman A, Han H, Collier JH. Switching the Immunogenicity of Peptide Assemblies Using Surface Properties. ACS Nano. 2016 Oct 25;10(10):9274-9286. doi: 10.1021/acsnano.6b03409. Epub 2016 Oct 3. PMID: 27680575; PMCID: PMC5704984.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.