Cynomolgus CD166/ALCAM (His Tag) recombinant protein

Cynomolgus CD166/ALCAM (His Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Activated leukocyte cell adhesion molecule (ALCAM)/Cluster of differentiation (CD166) is a type I transmembrane cell adhesion molecule belonging to the Ig superfamily and a ligand for CD6 that is expressed on T lymphocytes. The extracellular domain of ALCAM contains five Ig-like domains (three Ig-like C2-type domains and two Ig-like V-type domains), of which the amino-terminal V1 domain is essential for ligand binding and ALCAM-mediated cell aggregation. ALCAM mediates both heterophilic (ALCAM-CD6) and homophilic (ALCAM-ALCAM) cell-cell interactions. ALCAM/CD6 interaction plays a role in T cell development and T cell regulation, as well as in the binding of T- and B-cells to activated leukocytes. Recently, homophilic (ALCAM-ALCAM) adhesion was shown to play important roles in tight cell-to-cell interaction and regulation of stem cell differentiation. While expressed in a wide variety of tissues, ALCAM is usually restricted to subsets of cells involved in dynamic growth and/or migration, including neural development, branching organ development, hematopoiesis, immune response and tumor progression. And CD166 is regarded as a potential novel breast cancer indicator and therapeutic target.

Putative uncharacterized protein

MEMD; DM-GRASP; MUSC; AI853494; MGC27910; ALCAM; SC1; BEN; CD166

G7NZQ8

EGM_10314

Human Cells

A DNA sequence encoding the cynomolgus ALCAM (G7NZQ8) (Met1-Ala526) was expressed with a polyhistidine tag at the C-terminus.

Cynomolgus

The recombinant cynomolgus ALCAM comprises 510 amino acids and has a calculated molecular mass of 57.3 KDa. The apparent molecular mass of the protein is approximately 65-68 KDa respectively in SDS-PAGE.

> 95 % as determined by SDS-PAGE

Immobilized Cynomolgus ALCAM-His at 10 μg/ml (100 μl/well) can bind mouse CD6-Fc (Cat:500293), The EC₅₀ of mouse CD6-Fc (Cat:500293) is 0.07-0.15 μg/ml.