Pseudhymenochirin-2Pa peptide

Pseudhymenochirin-2Pa peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

H-GIFPIFAKLLGKVIKVASSLISKGRTE-OH

H-Gly-Ile-Phe-Pro-Ile-Phe-Ala-Lys-Leu-Leu-Gly-Lys-Val-Ile-Lys-Val-Ala-Ser-Ser-Leu-Ile-Ser-Lys-Gly-Arg-Thr-Glu-OH

27

95.12%

C₁₃₅H₂₃₀N₃₄O₃₄

2873.46

Synthetic

Powder

Active against S. aureus (MIC 5-10 uM), S. epidermidis (MIC 2.5-10 uM), E. faecalis (MIC 20 um), A. baumannii (10-20 uM), S. maltophilia (10-40 uM), and C. albicans (MIC 80 uM). The peptide is also activ eagainst select cancer cell lines (A549, MDA-231, and HT-29 (LC50 6-11.5 uM) and HUVEC (LC50 68 uM). It is also hemolytic (LC50 6.2 uM)(Mechkarska M et al. 2014). Updated 12/2014. Pseudhymenochirin-2Pa was found in skin secretions, Merlin's clawed frog, Pseudhymenochirus merlini, Africa.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides

• Conlon JM, Prajeep M, Mechkarska M, et al. Characterization of the host-defense peptides from skin secretions of Merlin's clawed frog Pseudhymenochirus merlini: insights into phylogenetic relationships among the Pipidae. Comp Biochem Physiol Part D Genomics Proteomics. 2013;8(4):352-7.
• Manzo G, Scorciapino MA, Srinivasan D, Attoub S, Mangoni ML, Rinaldi AC, Casu M, Flatt PR, Conlon JM. Conformational Analysis of the Host-Defense Peptides Pseudhymenochirin-1Pb and -2Pa and Design of Analogues with Insulin-Releasing Activities and Reduced Toxicities. J Nat Prod. 2015 Dec 24;78(12):3041-8.
• Mechkarska M, Attoub S, Sulaiman S, Pantic J, Lukic ML, Conlon JM. Anti-cancer, immunoregulatory, and antimicrobial activities of the frog skin host-defense peptides pseudhymenochirin-1Pb and pseudhymenochirin-2Pa. Regul Pept. 2014 Nov;194-195:69-76.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.