Pseudhymenochirin-1Pb peptide

Pseudhymenochirin-1Pb peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

IKIPSFFRNILKKVGKEAVSLIAGALKQS

Ile-Lys-Ile-Pro-Ser-Phe-Phe-Arg-Asn-Ile-Leu-Lys-Lys-Val-Gly-Lys-Glu-Ala-Val-Ser-Leu-Ile-Ala-Gly-Ala-Leu-Lys-Gln-Ser

29

97.5%

Synthetic

Powder

Active against S. aureus (MIC 5-10 uM), S. epidermidis (MIC 1.25-2.5 uM), E. faecalis (MIC 10 uM), K. pneumoniae (20 uM), P. aeruginosa (20 uM), A. baumannnii (5-10 uM), S. maltophilia (5-10 uM), E. coli (MIC 10 uM), and C. albicans (80 uM). The peptide is also activ eagainst select cancer cell lines (A549, MDA-231, HT-29, and HUVEC) (LC502.5-9.5 uM). It is also hemolytic (LC50 28 uM)(Mechkarska M et al. 2014). Updated 12/2014. Pseudhymenochirin-1Pb was found in skin secretions, Merlin's clawed frog, Pseudhymenochirus merlini, Africa.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Conlon JM, Prajeep M, Mechkarska M, et al. Characterization of the host-defense peptides from skin secretions of Merlin's clawed frog Pseudhymenochirus merlini: insights into phylogenetic relationships among the Pipidae. Comp Biochem Physiol Part D Genomics Proteomics. 2013;8(4):352-7.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.