Mouse HVEM/TNFRSF14 (His & Fc Tag) recombinant protein

Mouse HVEM/TNFRSF14 (His & Fc Tag) recombinant protein

• DataSheet
• Material Safety Data Sheets (MSDS)

Herpesvirus entry mediator (HVEM), also referred to as TNFRSF14, TR2 (TNF receptor-like molecule) and ATAR (another TRAF-associated receptor), is a member of type I transmembrane protein belonging to the TNF-receptor superfamily. It is expressed on many immune cells, including T and B cells, NK cells, monocytes, and neutrophils. Two TNF superfamily ligands lymphotoxin α (TNF-β) and LIGHT (TNFSF14) are identified as cellular ligands for HVEM and initiate the positive signaling. However, recent studies have revealed that HVEM is also involved in the unique inhibitory signaling pathway for T cells through activating tyrosine phosphorylation of the immunoreceptor tyrosine-based inhibitory motif (ITIM) in B and T lymphocyte attenuator (BTLA). HVEM provides a stimulatory signal following engagement with LIGHT (TNFSF14) on T cells. In contrast, it can also provide an inhibitory signal to T cells when it binds the B and T lymphocyte attenuator (BTLA), a ligand member of the Immunoglobulin (Ig) superfamily. Thus, HVEM may be viewed as a molecular switch, capable of facilitating both stimulatory and inhibitory cosignaling in T cells. Substantial evidence from both human disease and from experimental mouse models has indicated that dysregulation of the LIGHT-HVEM-BTLA cosignaling pathway can cause inflammation in the lung and in mucosal tissues.

Protein Tnfrsf14

RP3-395M20.6; TNFRS14; RP24-89N4.1; HVEA; MGC123499; HVEM; TR2; CD270; MGC123498; ATAR; LIGHTR; TNFRSF14

Q80WM9

Tnfrsf14

Human Cells

A DNA sequence encoding the extracellular domain (Met 1-Gln 206) of mouse HVEM (NP_849262.1) precursor was fused with C-terminal His-tagged Fc region of human IgG1 at the C-terminus.

Mouse

The recombinant mouse HVEM/Fc is a disulfide-linked homodimeric Protein after removal of the signal peptide. The reduced monomer consists of 415 amino acids and predicts a molecular mass of 46.4 kDa. By SDS-PAGE under reducing conditions, the apparent molecular mass of rmHVEM/Fc monomer is approximately 65 kDa due to the glycosylation.

> 90 % as determined by SDS-PAGE

Measured by its binding ability in a functional ELISA.
Immobilized mouse HVEM-Fch (Cat:500177）at 10 μg/mL (100 μl/well) can bind biotinylated mouse BTLA-Fc (Cat:504714)，The EC₅₀ of biotinylated mouse BTLA-Fc (Cat:504714) is 64-96 ng/mL.