Prosaptide TX14(A) peptide

Prosaptide TX14(A) peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-TALIDNNATEEILY-OH, Alanine-2 is D configuration

H-Thr-D-Ala-Leu-Ile-Asp-Asn-Asn-Ala-Thr-Glu-Glu-Ile-Leu-Tyr-OH

14

95.57%

C₆₉H₁₁₀N₁₆O₂₆

1579.69

Synthetic

Powder

Prosaptide TX14(A) is derived from the active region of saposin C, itself derived from proteolytic processing of prosaposin, a 517-amino acid residue protein and neurotropic factor. Prosaptide Tx 14(A) is a potent agonist of G protein-coupled receptors GPR37 (also known as Parkin-associated endothelin receptor-like receptor Pael-R) and GPR37L1 with EC50 values of 5 nM for GPR37L1 and 7 nM for GPR37. Prosaptide TX14(A) can protect primary astrocytes against oxidative stress, prolongs cell survival in both Schwann cells and oligodendrocytes and has anti-allodynic properties in a rat model of gp120-induced tactile allodynia.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Hiraiwa et al (1997) Cell death prevention, mitogen-activated protein kinase stimulation, and increased sulfatide concentrations in Schwann cells and oligodendrocytes by prosaposin and prosaptides. Proc Natl Acad Sci U S A. 94(9) 4778 PMID: 9114068
• Jolivalt et al (2008) Central action of prosaptide TX14(A) against gp120-induced allodynia in rats. Eur J Pain. 12(1) 76 PMID: 17466547
• Meyer et al (2013) GPR37 and GPR37L1 are receptors for the neuroprotective and glioprotective factors prosaptide and prosaposin. Proc.Natl.Acad.Sci.USA. 110 9529 PMID: 23690594

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.