PreNAC peptide

PreNAC peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-GVVHGVATVA-OH

H-Gly-Val-Val-His-Gly-Val-Ala-Thr-Val-Ala-OH

10

95.47%

C₄₀H₆₈N₁₂O₁₂

909.04

Synthetic

Powder

Recent research has shown that a specific protein called CypA plays a key role in the aggregation and toxicity of α-synuclein protein in the body. This protein catalyses the isomerisation of proline 128 in the C-terminal structural domain of α-synuclein, which contributes to its pathogenicity. Additionally, the hydrophobic sequence 47GVVHGVATVA56 forms a second CypA-binding site, which is crucial for the age-dependent aggregation and toxicity of αSyn in the nematode model of Hidradenitis elegans. By deleting this sequence, it is possible to prevent αSyn aggregation and toxicity. Furthermore, this sequence also plays a role in αSyn-mediated vesicle fusion by altering the conformational properties of the protein upon lipid binding. This region could be targeted to prevent αSyn aggregation in disease.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Doherty, C.P.A., Ulamec, S.M., Maya-Martinez, R. et al. A short motif in the N-terminal region of α-synuclein is critical for both aggregation and function. Nat Struct Mol Biol 27,249-259 (2020). https://doi.org/10.1038/s41594-020-0384-x

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.