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Product Name
PPAN antibody
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Description
PPAN Rabbit Polyclonal antibody. Positive IF detected in MCF-7 cells. Positive IHC detected in human lymphoma tissue. Positive WB detected in mouse heart tissue, rat heart tissue. Observed molecular weight by Western-blot: 55-58 kDa
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
BXDC3 antibody; Peter Pan homolog antibody; peter pan homolog (Drosophila) antibody; PPAN antibody; SSF antibody; Ssf 1 antibody; SSF1 antibody; SSF2 antibody; Suppressor of SWI4 1 homolog antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of PPAN recombinant protein (Accession Number: NM_020230). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
mouse heart tissue were subjected to SDS PAGE followed by western blot with Catalog No:114079(PPAN antibody) at dilution of 1:800
Immunohistochemical of paraffin-embedded human lymphoma using Catalog No:114079(PPAN antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of MCF-7 cells, using Catalog No:114079 and FITC-labeled donkey anti-rabbit IgG (green). Blue pseudocolor = DAPI (fluorescent DNA dye).
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Background
PPAN (or SSF, SSF1 or SSF2) gene is widely expressed, and encodes peter pan homolog protein which may be localized in nucleus. Two isoforms are produced through alternative splicing. So far the characterization of this protein is largely unknown, multiple phosphorylation sites has been revealed based on high-throughput phosphorylation analysis. This antibody is raised against the C-terminal of huaman PPAN and recognize bands around 55 kDa.
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References
- Pfister AS, Keil M, Kühl M. The Wnt Target Protein Peter Pan Defines a Novel p53-independent Nucleolar Stress-Response Pathway. The Journal of biological chemistry. 290(17):10905-18. 2015.
- Bugner V, Tecza A, Gessert S, Kühl M. Peter Pan functions independently of its role in ribosome biogenesis during early eye and craniofacial cartilage development in Xenopus laevis. Development (Cambridge, England). 138(11):2369-78. 2011.
- Tecza A, Bugner V, Kühl M, Kühl SJ. Pescadillo homologue 1 and Peter Pan function during Xenopus laevis pronephros development. Biology of the cell / under the auspices of the European Cell Biology Organization. 103(10):483-98. 2011.
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