Potative neuropeptide 2

Potative neuropeptide 2

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-GSGTKVGGAGAATKVVTKSGS-NH2

H-Gly-Ser-Gly-Thr-Lys-Val-Gly-Gly-Ala-Gly-Ala-Ala-Thr-Lys-Val-Val-Thr-Lys-Ser-Gly-Ser-NH2

21

95.25%

C₇₅H₁₃₅N₂₅O₂₇

1819.01

Synthetic

Powder

Putative neuropeptide 2 is a neuropeptide that is processed from the BmK5 precursor protein in the silkworm(Bombyx mori), Bombyx mori.
Putative neuropeptide 2 is one of the several neuropeptides produced by the BmK5 precursor protein and is transported to the corpora cardiaca-corpora allata (CC-CA) and released into the hemolymph.
The antibody raised against Putative neuropeptide 2, which has an amidated C-terminus, recognizes the axons connecting the BmK5 antibody-positive cells to the CC-CA. This suggests that at least one of the mature forms of BmK5 includes amino acid residues corresponding to Putative neuropeptide 2 or may be amidated Putative neuropeptide 2 itself.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Mitsumasu K, Tanaka Y, Niimi T, Yamashita O, Yaginuma T. Novel gene encoding precursor protein consisting of possible several neuropeptides expressed in brain and frontal ganglion of the silkworm, Bombyx mori. Peptides. 2009;30(7):1233-40.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.