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Product Name
POP5 Polyclonal Antibody
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Description
Polyclonal antibody to POP5
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Tested applications
WB
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Species reactivity
Human, Rat
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Alternative names
POP5 antibody; HSPC004 antibody; RPP2 antibody; RPP20 antibody; hPop5 antibody; ribonuclease P/MRP protein subunit POP5 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide corresponding to a sequence within amino acids 50-150 of human POP5 (NP_057002.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
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Validations
Western blot - POP5 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using POP5 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.
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Background
Component of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends. Also a component of RNase MRP.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"