Pis1 peptide

Pis1 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

FIHHIIGWISHGVRAIHRAIHG

Phe-Ile-His-His-Ile-Ile-Gly-Trp-Ile-Ser-His-Gly-Val-Arg-Ala-Ile-His-Arg-Ala-Ile-His-Gly

22

97.1%

Synthetic

Powder

It is likely that the last Glycine can be converted to C-terminal amidation (e.g., GAD-1) (McDonald M et al., 2015). These authors found that both Gad-1 and Gad-2 demonstrated concentration-dependent cytotoxicity against LLC cells, HEY ovarian cancer cells, and PC3 prostate cancer cells. Ruangsri et al. found it active against Gram-positive bacteria M. luteus (M. lysodeikticus) ATCC 4698, P. citreus NCIMB 1493, C. glutamicum ATCC 13032, S. aureus ATCC 9144 (MIC , 0.04-5 uM) and Gram-negative bacteria E. coli ATCC 25922, V. anguillarum NCIMB 2133, V. anguillarum NCIMB 2129, Y. ruckeric NCIMB 2196, Y. ruckeric VI-3629, and P. immobilisc NCIMB 308 (MIC 0.3-10 uM). The C-terminal 17 residues are helical in SDS. Updated 4/2015. Pis1 has Antibacterial activity. The source of Pis1 is Atlantic cod, Gadus morhua L..

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Ruangsri J, Salger SA, Caipang CM, Kiron V, Fernandes JM. Differential expression and biological activity of two piscidin paralogues and a novel splice variant in Atlantic cod (Gadus morhua L.). Fish Shellfish Immunol. 2012;32(3):396-406.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.