Phosphatase inhibitor pentapeptide 2

Phosphatase inhibitor pentapeptide 2

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

ERGMT

Glu-Arg-Gly-Met-Thr

5

95.9%

Synthetic

Powder

The phosphatase inhibitor pentapeptide 2 (NH2-ERGMT-COOH) is a specific pentapeptide inhibitor of Rap phosphatases in Bacillus subtilis. It is generated from the carboxy-terminal end of a precursor protein encoded by phr genes. The active pentapeptide form inhibits the phosphatase activity of Rap proteins, which dephosphorylate the Spo0F~P intermediate response regulator in the sporulation phosphorelay. This inhibition is part of a complex export-import processing pathway that regulates the production of the Phr pentapeptides. The structural organization of the Phr precursor suggests a SecA-dependent secretion pathway, but processing to the active pentapeptide occurs through a single cleavage event at the peptide bond on the amino-terminal end of the pentapeptide. This cleavage is independent of type I signal peptidase activity. The pentapeptide sequence is required to be at the carboxy-terminal end of the precursor, and an alanine residue at the -1 position preceding the pentapeptide is essential for correct processing.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Stephenson S, Mueller C, Jiang M, Perego M. Molecular analysis of Phr peptide processing in Bacillus subtilis. J Bacteriol. 2003;185(16):4861-71.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.