Phe-Pro-Ala-pNA peptide

Phe-Pro-Ala-pNA peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-FPA-pNA

H-Phe-Pro-Ala-pNA

3

95.67%

C₂₃H₂₇N₅O₅

453.49

201738-99-0

71358542

Synthetic

Powder

Phe-Pro-Ala-pNA (Phe-Pro-Ala-4-nitroanilide) is a synthetic peptide substrate used to test the exoproteolytic activity of the heterologously expressed SedB, SedC, and SedD proteins from Aspergillus fumigatus. These three proteins had no endoproteolytic activity but released pNA very efficiently when Phe-Pro-Ala-pNA or Ala-Ala-Phe-pNA was used as the substrate. The optimal pH for SedB and SedC activity with this substrate was 6.0, while for SedD it was 5.0.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Reichard U, Léchenne B, Asif AR, Streit F, Grouzmann E, Jousson O, Monod M. Sedolisins, a new class of secreted proteases from Aspergillus fumigatus with endoprotease or tripeptidyl-peptidase activity at acidic pHs. Appl Environ Microbiol. 2006 Mar;72(3):1739-48. doi: 10.1128/AEM.72.3.1739-1748.2006. PMID: 16517617; PMCID: PMC1393174.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.