Periplanetasin-4 peptide (TFA removed)

Periplanetasin-4 peptide (TFA removed)

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-LRHKVYGYCVLGP-NH2

H-Leu-Arg-His-Lys-Val-Tyr-Gly-Tyr-Cys-Val-Leu-Gly-Pro-NH2

13

97.67%

C₇₀H₁₁₀N₂₀O₁₅S

1503.82

Synthetic

Powder

Periplanetasin-4 is a novel antimicrobial peptide derived from the American Cockroach (Periplaneta americana). Periplanetasin-4 has diverse effects on fungal cells, including antimicrobial activity, induction of cell death with unique apoptotic and necrotic features, disruption of mitochondrial and vacuolar functions, and modulation of intracellular signaling pathways.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Lee H, Hwang JS, Lee DG. Periplanetasin-4, a novel antimicrobial peptide from the cockroach, inhibits communications between mitochondria and vacuoles. Biochem J. 2019 Apr 26;476(8):1267-1284. doi: 10.1042/BCJ20180933. PMID: 30962256.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.