PepW peptide

PepW peptide

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-WWWA(Dab)YGL(Dab)LL(Dab)Y(Dab)(Dab)WY-OH

H-Trp-Trp-Trp-Ala-(Dab)-Tyr-Gly-Leu-(Dab)-Leu-Leu-(Dab)-Tyr-(Dab)-(Dab)-Trp-Tyr-OH

17

95.2%

C₁₀₉H₁₃₅N₂₁O₂₁

2260.61

Synthetic

Powder

PepW binding to capsule and the subsequent structure loss leads to capsule-peptide aggregation that distorts or removes the capsule, allowing the remaining free peptide to disrupt the bacterial membrane . This mechanism for antimicrobial peptide activity toward capsulated K. pneumoniae offers a ray of light in the fight for effective therapeutics toward this extremely resistant species.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Fleeman RM, Macias LA, Brodbelt JS, Davies BW. Defining principles that influence antimicrobial peptide activity against capsulated Klebsiella pneumoniae. Proc Natl Acad Sci U S A. 2020 Nov 3;117(44):27620-27626. doi: 10.1073/pnas.2007036117. Epub 2020 Oct 21. PMID: 33087568; PMCID: PMC7959497.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.