Peptide5

Peptide5

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-VDCFLSRPTEKT-OH

H-Val-Asp-Cys-Phe-Leu-Ser-Arg-Pro-Thr-Glu-Lys-Thr-OH

12

95.24%

C₆₀H₉₈N₁₆O₂₀S

1395.57

Synthetic

Powder

Peptide5, also known as connexin43 mimetic peptide 5, was designed to bind to extracellular regions of the connexin43 (Cx43) protein, and is a blocker of Connexin43 hemichannels. Connexin43 hemichannels in spinal cord astrocytes are implicated in the maintenance of neuropathic pain following peripheral nerve injury and Peptide 5 can reduce swelling, astrogliosis, neuroinflammation and neuronal cell death following spinal cord injury. Peptide5 exhibits analgesic effects in models of neuropathic pain.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• O'Carroll et al (2008) Connexin 43 mimetic peptides reduce swelling, astrogliosis and neuronal cell death after spinal cord injury. Cell.Commun.Adhes. 15 27 PMID: 18649176
• Mao et al (2017) Characterisation of Peptide5 systemic administration for treating traumatic spinal cord injured rats. Exp.Brain.Res. 235 3033 PMID: 28725925
• Tonkin et al (2017) Attenuation of mechanical pain hypersensitivity by treatment with Peptide5, a connexin-43 mimetic peptide, involves inhibition of NLRP3 inflammasome in nerve-injured mice. Exp.Neurol. 300 1 PMID: 29055716

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.