Peptide AN5-1

Peptide AN5-1

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

YSKSLPLSVLNP

Tyr-Ser-Lys-Ser-Leu-Pro-Leu-Ser-Val-Leu-Asn-Pro

12

98.2%

Synthetic

Powder

bactericidal, inducing cell lysis in E. coli ATCC 29522 and S. aureus, and non-cell lysis in both S. marcescens and B. cereus ATCC 14579. MIC 4-8 ug/ml against S. enteritidis ATCC 13076 and E. coli; 16-32 ug/ml against Enterobacter spp.,S. marcescens, L. plantarum ATCC 8014, B. subtilis; 64 ug/ml against B. cereus ATCC 14579 and S. aureus; and 128 ug/ml against B. anthracis. This peptide can disrupt bacterial membranes and bind to DNA (Yi T et al., 2014). Updated 10/2014. Peptide AN5-1 has Antibacterial activity. The source of Peptide AN5-1 is Paenibacillus alvei AN5.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Alkotaini B, Anuar N, Kadhum AA, Sani AA. Detection of secreted antimicrobial peptides isolated from cell-free culture supernatant of Paenibacillus alvei AN5. J Ind Microbiol Biotechnol. 2013;40(6):571-9.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.