Pep27 peptide

Pep27 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

MRKEFHNVLSSGQLLADKRPARDYNRK

Met-Arg-Lys-Glu-Phe-His-Asn-Val-Leu-Ser-Ser-Gly-Gln-Leu-Leu-Ala-Asp-Lys-Arg-Pro-Ala-Arg-Asp-Tyr-Asn-Arg-Lys

27

97.8%

Synthetic

Powder

Pep27anal2 characterized substituting (2R-->W), (4E-->W), (11S-->W) and (13Q-->W) in native Pep27, exhibited greater hydrophobicity and anticancer activity than Pep27 and other analogues. The IC50 values of Pep27anal2 were approximately 10 - 30 microM in a number of cell lines (AML-2, HL-60, Jurkat, MCF-7 and SNU-601). The antibacterial activities of Pep27 are fundamentally attributable to a physiological change by activated phosphatase activity (Biochem Biophys Res Commun. 2007 Nov 23;363(3):806-10). Pep27 has Anticancer activity. Pep27 was found in Streptococcus pneumoniae.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.