PDSGR peptide

PDSGR peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

Ac-PDSGR-OH

Ac-Pro-Asp-Ser-Gly-Arg-OH

5

95.68%

C₂₂H₃₆N₈O₁₀

572.56

Synthetic

Powder

PDSGR peptide is a bioactive short peptide designed for nerve tissue engineering research. It features an acyl-blocked N-terminal, enhancing aggregation tendency, while its hydrophilic C-terminal with the PDSGR sequence remains open. However, its solubility in 20 mM HEPES buffer at pH 6 is poor due to the presence of a proline residue, which also leads to weak self-assembly ability, forming only a few small peptide segments. Despite these limitations, it is part of the exploration in developing effective scaffolds for nerve tissue engineering, with potential for further modifications or combinations to unlock its functionality in promoting cell behaviors and neural tissue regeneration.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Sun W, Taylor CS, Gao Z, Gregory DA, Haycock JW, Zhao X. Co-assembling bioactive short peptide nanofibers coated silk scaffolds induce neurite outgrowth of PC12 cells. Int J Biol Macromol. 2024 Oct;278(Pt 2):134774. doi: 10.1016/j.ijbiomac.2024.134774. Epub 2024 Aug 16. PMID: 39154681.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.