Recombinant PDE12 protein (His tag)

• DataSheet
• COA (Batch NP456410)
• Material Safety Data Sheets (MSDS)

Recombinant Human 2',5'-phosphodiesterase 12 (PDE12) fragment (residues 122-609) - a highly purified protein preparation ideal for studying mitochondrial deadenylase function and the 2-5A antiviral pathway. This stable 488-amino acid fragment maintains key enzymatic activities while offering enhanced solubility for structural and biochemical applications.

2',5'-phosphodiesterase 12 (PDE12) is a mitochondrial enzyme that plays a critical role as a negative regulator of the 2-5A system, a major pathway for antiviral and antitumor functions induced by interferons. The enzyme cleaves both 2',5'-phosphodiester bonds in triphosphorylated oligoadenylates (2-5A) and 3',5'-phosphodiester bonds of oligoadenylates, degrading 2-5A to produce AMP and ATP. By suppressing cellular 2-5A levels, PDE12 modulates antiviral responses, and its inhibition has been shown to decrease viral replication in cultured cells. The protein localizes to the mitochondrial matrix, where it performs its regulatory functions in innate immunity pathways.

MSKIKVVKLY YREEAVAEDV LNVDAWQDGA VLQIGDVKYK VERNPPAFTE LQLPRYIMAG FPVCPKLSLE FGDPASSLFR
WYKEAKPGAA EPEVGVPSSL SPSSPSSSWT ETDVEERVYT PSNADIGLRL KLHCTPGDGQ RFGHSRELES VCVVEAGPGT
CTFDHRHLYT KKVTEDALIR TVSYNILADT YAQTEFSRTV LYPYCAPYAL ELDYRQNLIQ KELTGYNADV ICLQEVDRAV
FSDSLVPALE AFGLEGVFRI KQHEGLATFY RKSKFSLLSQ HDISFYEALE SDPLHKELLE KLVLYPSAQE KVLQRSSVLQ
VSVLQSTKDS SKRICVANTH LYWHPKGGYI RLIQMAVALA HIRHVSCDLY PGIPVIFCGD FNSTPSTGMY HFVINGSIPE
DHEDWASNGE EERCNMSLTH FFKLKSACGE PAYTNYVGGF HGCLDYIFID LNALEVEQVI PLPSHEEVTT HQALPSVSHP
SDHIALVCDL KWKLEHHHHH H