Parkerin peptide

Parkerin peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

GWANTLKNVAGGLCKITGAA

Gly-Trp-Ala-Asn-Thr-Leu-Lys-Asn-Val-Ala-Gly-Gly-Leu-Cys-Lys-Ile-Thr-Gly-Ala-Ala

20

97%

Synthetic

Powder

Active against Gram+ S. aureus ATCC 2592 and Enterococcus faecium and Gram- Acinetobacter baumannii (MIC 37.5 ug/ml) and fungus C. albicans ATCC 2002 (MIC 75 ug/ml). Its antioxidant activity could also be important for the survival of this frog in Xizang (Tibet) plateau habitat. The peptide showed a poor hemolytic activity (<2.4%) at 80 ug/ml. The peptide is random in water but helical upon association with membrane-mimetic TFE or SDS micelles. Parkerin has Antimicrobial activity. The source of Parkerin is (China, Asia) Xizang plateau frog, Nanorana parkeri.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.