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Product Name
Pan Acetylation antibody
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Description
Pan Acetylation Mouse Monoclonal antibody. Positive WB detected in HeLa cells. Positive IF detected in MDCK cells. Observed molecular weight by Western-blot: 17 kDa
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Tested applications
ELISA, WB, IF
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Species reactivity
Human, Mouse, Rat, Dog; other species not tested.
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Alternative names
Acetylation antibody; Acetylation Lysine antibody; Pan Acetylation antibody
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Isotype
Mouse IgG1
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Preparation
This antibody was obtained by immunization of Peptide. Purification method: Protein G purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:107451(Pan Acetylation Antibody) at dilution of 1:1500
Immunofluorescent analysis of (10% Formaldehyde) fixed MDCK cells using (Pan Acetylation Antibody) at dilution of 1:150 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)
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Background
Acetylation and De-acetylation are the important reaction in epigenetic. Many proteins, especially Histone, are with Acetylated of Lysine. This antibody is used to detect the Acetylation of Lysine. The ~17 kDa in our detection is Acetylated Histone.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"