PAM-5 peptide

PAM-5 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

1 Vial

H-KWKWRPLKRKLVLRM-OH

H-Lys-Trp-Lys-Trp-Arg-Pro-Leu-Lys-Arg-Lys-Leu-Val-Leu-Arg-Met-OH

15

95.96

C₉₇H₁₆₄N₃₀O₁₆S

2038.59

Synthetic

PAM-5 is a potent antibacterial peptide that was developed through phage display selection and computer-assisted modification. It has enhanced antibacterial features, as demonstrated by its ability to cause membrane disruption and permeabilization of bacteria, bind bacterial DNA, and kill the bacteria rapidly in 10 minutes based on time-kill assay results. PAM-5 also exhibits stability in human plasma via ex vivo assays while being non-cytotoxic towards Vero cells at all concentrations tested for the antibacterial assays.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides

• Yuen HL, Chan SY, Ding YE, Lim S, Tan GC, Kho CL. Development of a Novel Antibacterial Peptide, PAM-5, via Combination of Phage Display Selection and Computer-Assisted Modification. Biomolecules. 2023; 13(3):466. https://doi.org/10.3390/biom13030466

Trifluoroacetic acid (TFA) is a strong acid, which is commonly used to cleave synthesized peptides from solid-phase resins and is also used to improve HPLC performance in the peptide purification step. By default, custom peptides are delivered as lyophilized TFA salts, and can contain as much as 10-45% TFA.

TFA in custom peptides can cause inexplicable discrepancies in subsequent assay data. For instance, TFA in nM concentrations has been shown to interfere with cellular assays, inhibiting cellular proliferation in some instances, and increasing cell viability in others . It has also been found to be an unintended allosteric modulator of the glycine receptor, GlyR.

TFA Removal Service is recommended for:

• Peptides that will be used in cellular assays
• Peptides that will be used as APIs or in manufactured products
• For hydrophilic peptides containing numerous basic residues