PAM-5 peptide

PAM-5 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

H-KWKWRPLKRKLVLRM-OH

H-Lys-Trp-Lys-Trp-Arg-Pro-Leu-Lys-Arg-Lys-Leu-Val-Leu-Arg-Met-OH

15

95.96%

C₉₇H₁₆₄N₃₀O₁₆S

2038.59

Synthetic

Powder

PAM-5 is a potent antibacterial peptide that was developed through phage display selection and computer-assisted modification. It has enhanced antibacterial features, as demonstrated by its ability to cause membrane disruption and permeabilization of bacteria, bind bacterial DNA, and kill the bacteria rapidly in 10 minutes based on time-kill assay results. PAM-5 also exhibits stability in human plasma via ex vivo assays while being non-cytotoxic towards Vero cells at all concentrations tested for the antibacterial assays.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides

• Yuen HL, Chan SY, Ding YE, Lim S, Tan GC, Kho CL. Development of a Novel Antibacterial Peptide, PAM-5, via Combination of Phage Display Selection and Computer-Assisted Modification. Biomolecules. 2023; 13(3):466. https://doi.org/10.3390/biom13030466

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.