Pal-KTTKS peptide

Pal-KTTKS peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

Palmitoyl-KTTKS-OH

Palmitoyl-Lys-Thr-Thr-Lys-Ser-OH

5

95.25%

C₃₉H₇₅N₇O₁₀

802.05

Synthetic

Powder

Pal-KTTKS, a lipidated subfragment of type 1 pro-collagen (residues 212–216), plays a role in fibroblast contractility. Pal-KTTKS reduces α-SMA expression and trans-differentiation of fibroblasts to myofibroblast. KTTKS is a small, highly specific biologically active peptide which has been reported to produce stimulation of elastin and collagens, specifically types I and III.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Park H, An E, Cho Lee AR. Effect of Palmitoyl-Pentapeptide (Pal-KTTKS) on Wound Contractile Process in Relation with Connective Tissue Growth Factor and α-Smooth Muscle Actin Expression. Tissue Eng Regen Med. 2017 Jan 19;14(1):73-80. doi: 10.1007/s13770-016-0017-y. PMID: 30603464; PMCID: PMC6171572.
• Abu Samah NH, Heard CM. Topically applied KTTKS: a review. Int J Cosmet Sci. 2011 Dec;33(6):483-90. doi: 10.1111/j.1468-2494.2011.00657.x. Epub 2011 May 3. PMID: 21535443.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.