CIGB-552 peptide

CIGB-552 peptide

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

Ac-HARIK-{d-Pro}-TFRR-{d-Leu}-KWKYKGKFW

Ac-His-Ala-Arg-Ile-Lys-D-Pro-Thr-Phe-Arg-Arg-D-Leu-Lys-Trp-Lys-Tyr-Lys-Gly-Lys-Phe-Trp

20

97.48%

C₁₃₁H₁₉₉N₃₉O₂₃

2689.23

1630763-23-3

Synthetic

Powder

CIGB-552 is a second-generation anticancer peptide derived from L-2, which was identified by alanine scanning of the Limulus LALF32–51 region. It increases cellular COMMD1 levels, promotes RelA ubiquitination, and inhibits NF-κB-mediated antiapoptotic signaling—an effect abolished by COMMD1 knockdown. It also decreases antioxidant capacity and induces protein and lipid peroxidation in tumor cells. Further studies confirmed that COMMD1 is essential for its mechanism, enabling negative modulation of NF-κB and HIF-1 pathways, with anti-inflammatory and anti-angiogenic effects. CIGB-552 reduces tumor size and extends survival in tumor-bearing mice.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• [1] Daghero, Hellen et al. “The Anticancer Peptide CIGB-552 Exerts Anti-Inflammatory and Anti-Angiogenic Effects through COMMD1.” Molecules (Basel, Switzerland) vol. 26,1 152. 31 Dec. 2020, doi:10.3390/molecules26010152
• [2] Gomez Rodriguez, Yolanda et al. “Synergic effect of anticancer peptide CIGB-552 and Cisplatin in lung cancer models.” Molecular biology reports vol. 49,4 (2022): 3197-3212. doi:10.1007/s11033-022-07152-3
• [3] Fernández Massó, Julio R et al. “The Antitumor Peptide CIGB-552 Increases COMMD1 and Inhibits Growth of Human Lung Cancer Cells.” Journal of amino acids vol. 2013 (2013): 251398. doi:10.1155/2013/251398

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.