p206 peptide

p206 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-TYEIAPVFVLLEYVT-OH

H-Thr-Tyr-Glu-Ile-Ala-Pro-Val-Phe-Val-Leu-Leu-Glu-Tyr-Val-Thr-OH

15

95.8%

C₈₆H₁₂₉N₁₅O₂₄

1757.02

Synthetic

Powder

Peptide p206 is an immunodominant epitope of glutamic acid decarboxylase 65 (GAD65), which is a major pancreatic antigen targeted by self-reactive T cells in type I diabetes mellitus. The paper suggests that p206-specific T cells may play a protective role in diabetes pathogenesis by regulating pathogenic T cell responses. The study was conducted in NOD mice expressing a transgenic T cell antigen receptor (TCR) specific for p206, and the results showed that p206-specific T cells delayed the onset of diabetes when adoptively transferred along with diabetogenic T cells. These observations have important implications for the design of antigen-specific immunotherapies for the treatment and prevention of type I diabetes. However, further research is needed to explore the potential clinical applications of p206-specific T cells in the treatment of type I diabetes in humans.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Kim SK, Tarbell KV, Sanna M, Vadeboncoeur M, Warganich T, Lee M, Davis M, McDevitt HO. Prevention of type I diabetes transfer by glutamic acid decarboxylase 65 peptide 206-220-specific T cells. Proc Natl Acad Sci U S A. 2004 Sep 28;101(39):14204-9. doi: 10.1073/pnas.0405500101. Epub 2004 Sep 20. PMID: 15381770; PMCID: PMC521137.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.