P10 peptide

P10 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

H-LAREYKKIVEKLKRWLRQVLRTLR-OH

H-Leu-Ala-Arg-Glu-Tyr-Lys-Lys-Ile-Val-Glu-Lys-Leu-Lys-Arg-Trp-Leu-Arg-Gln-Val-Leu-Arg-Thr-Leu-Arg-OH

24

95.73%

C₁₄₂H₂₄₇N₄₅O₃₂

3096.75

Synthetic

Powder

Shorter peptides derived from LL-37, such as P10 (LAREYKKIVEKLKRWLRQVLRTLR), have also shown antimicrobial activity. P10 was more effective at killing the methicillin-resistant S. aureus (MRSA) strain LUH14616 than LL-37, and it could eradicate MRSA strains LUH14616 and LUH15051 from thermally wounded human skin equivalents in vitro without showing toxicity towards the fibroblasts and keratinocytes within the tissue models.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides

• Haisma EM et al (2014) LL-37-derived peptides eradicate multidrug-resistant Staphylococcus
• aureus from thermally wounded human skin equivalents. Antimicrob Agents Chemother
• 58:4411. https://doi.org/10.1128/AAC.02554-14

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.