Orexin B (human) peptide

Orexin B (human) peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

RSGPPGLQGRLQRLLQASGNHAAGILTM-NH2

H-Arg-Ser-Gly-Pro-Pro-Gly-Leu-Gln-Gly-Arg-Leu-Gln-Arg-Leu-Leu-Gln-Ala-Ser-Gly-Asn-His-Ala-Ala-Gly-Ile-Leu-Thr-Met-NH2

28

95.7%

C₁₂₃H₂₁₂N₄₄O₃₅S

2899.38

205640-91-1

Synthetic

Powder

Like orexin A, orexin B is a hypothalamic neuropeptide regulating the feeding behavior. Central administration of this peptide to rats stimulated food consumption in a similar manner to orexin A, but the effect did not last as long as that of orexin A. The affinity of orexin B for the OX1 receptor is significantly lower (IC50 = 240 nM) than that of orexin A. Binding affinities for the OX2 receptor are similar for orexin A and B (IC50

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• M.W.Schwartz, Nat. Med., 4, 385 (1998)
• T.Sakurai et al., Cell, 92, 573 (1998)
• M.R.Jain et al., Regul. Peptides, 87, 19 (2000)

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.