DBI 43-50 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

The peptide RPGLLDLK is a synthetic fragment corresponding to residues 43-50 of the octadecaneuropeptide (ODN), which is itself a brain-derived processing product of diazepam-binding inhibitor (DBI). This octapeptide, along with the closely related fragment DRPGLLDLK (DBI 42-50), represents the C-terminal sequence of ODN. Studies involving intrahippocampal injection in rats have demonstrated that RPGLLDLK can induce electroencephalographic alterations characterized by patterns of limbic seizures, including high-frequency activity and synchronized spike-wave discharges. The epileptogenic activity of this peptide fragment is notably antagonized by PK 11195, a selective ligand for a peripheral-type benzodiazepine recognition site linked to the GABA_A receptor complex, but is unaffected by antagonists of central benzodiazepine or NMDA-type glutamate receptors.

• Patteux C, Foucout L, Bohn P, Dupas G, Leprince J, Tonon MC, Dehouck B, Marsais F, Papamicaël C, Levacher V Solid phase synthesis of a redox delivery system with the aim of targeting peptides into the brain Org Biomol Chem 2006 Mar 7;4(5):817-25
• Vezzani A, Serafini R, Stasi MA, Samanin R, Ferrarese C. Epileptogenic activity of two peptides derived from diazepam binding inhibitor after intrahippocampal injection in rats. Epilepsia. 1991 Sep-Oct;32(5):597-603. doi: 10.1111/j.1528-1157.1991.tb04698.x. PMID: 1655399.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.