Ocellatin-V1 peptide

Ocellatin-V1 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

1 Vial

GVVDILKGAGKDLLAHALSKLSEKV

Gly-Val-Val-Asp-Ile-Leu-Lys-Gly-Ala-Gly-Lys-Asp-Leu-Leu-Ala-His-Ala-Leu-Ser-Lys-Leu-Ser-Glu-Lys-Val

25

97.6

Synthetic

The very low antimicrobial potency (MIC>200 uM) against E. coli and S. aureus associated with the peptides is probably a consequence of their lack of amphipathicity and reduced cationicity compared with active members of the ocellatin family from related species. Ocellatin-V1 has Antimicrobial activity. The source of Ocellatin-V1 is smooth-skinned ditch frog, Leptodactylus validus, South America.

Ideally Ocellatin-V1 peptide should be stored in a freezer at or below -9C. Ocellatin-V1 peptide should be refrigerated after reconstitution. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides

• King JD, Leprince J, Vaudry H, Coquet L, Jouenne T, Conlon JM. Purification and characterization of antimicrobial peptides from the Caribbean frog, Leptodactylus validus (Anura: Leptodactylidae). Peptides. 2008;29(8):1287-92.

Trifluoroacetic acid (TFA) is a strong acid, which is commonly used to cleave synthesized peptides from solid-phase resins and is also used to improve HPLC performance in the peptide purification step. By default, custom peptides are delivered as lyophilized TFA salts, and can contain as much as 10-45% TFA.

TFA in custom peptides can cause inexplicable discrepancies in subsequent assay data. For instance, TFA in nM concentrations has been shown to interfere with cellular assays, inhibiting cellular proliferation in some instances, and increasing cell viability in others . It has also been found to be an unintended allosteric modulator of the glycine receptor, GlyR.

TFA Removal Service is recommended for:

• Peptides that will be used in cellular assays
• Peptides that will be used as APIs or in manufactured products
• For hydrophilic peptides containing numerous basic residues