H2N2 Nucleoprotein (His Tag)

H2N2 Nucleoprotein (His Tag)

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Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals and is responsible of the active RNP import into the nucleus through the cellular importin alpha/beta pathway. Later in the infection, nucleus export of RNP are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that the nucleoprotein binds directly exportin-1 (XPO1) and plays an active role in RNP nuclear export. M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmask nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus.

Nucleoprotein

NA; NEURAMINIDASE; NANH; NEDA; SIALIDASE

P03466

NP

Baculovirus-Insect Cells

A DNA sequence encoding the Influenza A virus (A/Ann Arbor/6/1960 (H2N2)) nucleoprotein (AAM75159.1) (Met 1-Asn 498) was fused with a polyhistidine tag at the C-terminus.

H2N2

The recombinant Influenza A virus (A/Ann Arbor/6/1960(H2N2)) nucleoprotein comprises 508 amino acids and has a predicted molecular mass of 57 kDa. It migrates as an approximately 52 kDa band in SDS-PAGE under reducing conditions.

> 95 % as determined by SDS-PAGE