H7N9 Nucleoprotein (His Tag)

H7N9 Nucleoprotein (His Tag)

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Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals and is responsible of the active RNP import into the nucleus through the cellular importin alpha/beta pathway. Later in the infection, nucleus export of RNP are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that the nucleoprotein binds directly exportin-1 (XPO1) and plays an active role in RNP nuclear export. M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmask nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus (By similarity).

Nucleoprotein

SIALIDASE; NEURAMINIDASE; NANH; NEDA; NA

R4NE06

NP

Baculovirus-Insect Cells

A DNA sequence encoding the Influenza A virus (A/Shanghai/2/2013(H7N9)) nucleoprotein protein (AGL44439.1) (Met1-Asn498) was expressed with a C-terminal polyhistidine tag.

H7N9

The recombinant nucleoprotein protein of Influenza A virus (A/Shanghai/1/2013(H7N9)) comprises 509 amino acids and has a predicted molecular mass of 57.8 kDa. The apparent molecular mass of the protein is approximately 57 kDa in SDS-PAGE under reducing conditions.

> 95 % as determined by SDS-PAGE